Journal: eLife

Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons

doi: 10.7554/eLife.112083

Figure Lengend Snippet: ( A ) Experimental timeline. ( B ) Representative confocal images at D4, D10, D30, and D45 showing that GFP is not co-labeled with TUJ1 or MAP2 in the brain. ( C ) Quantification of TUJ1 + GFP + double-positive cells among TUJ1 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( D ) Quantification of MAP2 + GFP + double-positive cells among MAP2 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( E ) Relative gene expression level at D18. N = 4 mice for each group. Data are presented as mean ± SD. Each data point represents one mouse.

Article Snippet: The cell pellet was then resuspended in 90 μl of 0.5% BSA in DPBS and incubated with 10 μl of anti-CD11b magnetic beads (Miltenyi, Cat#: 130-097-142) for 30 min at 4°C.

Techniques: Confocal, Labeling, Gene Expression, Magnetic Cell Separation, Real-time Polymerase Chain Reaction

Journal: eLife

Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons

doi: 10.7554/eLife.112083

Figure Lengend Snippet: Using qPCR verify the relative expression of Neurod1 .

Article Snippet: The cell pellet was then resuspended in 90 μl of 0.5% BSA in DPBS and incubated with 10 μl of anti-CD11b magnetic beads (Miltenyi, Cat#: 130-097-142) for 30 min at 4°C.

Techniques: Expressing, Gene Expression